pCGTAG vector (V008534)

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LOCUS       40924_10601       12907 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway adapted binary vector pCGTAG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Zhang C, Gong FC, Lambert GM, Galbraith DW.
  TITLE     Cell type-specific characterization of nuclear DNA contents within 
            complex tissues and organs
  JOURNAL   Plant Methods 1 (1), 7 (2005)
  PUBMED    16270943
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Zhang C, Galbraith DW.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2006) Department of Plant Sciences, University of 
            Arizona, 303 Forbes Building, Tucson, AZ 85721-0036, USA
REFERENCE   3  (bases 1 to 12907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods"; date: "2005"; volume: "1"; issue: "1"; pages: "7"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-JAN-2006) Department of Plant Sciences, University of Arizona, 
            303 Forbes Building, Tucson, AZ 85721-0036, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..129
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        155..185
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             239..916
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1239..1541
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1585..1709)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     5'UTR           1715..1850
                     /note="translation leader sequence; 5'UTR from tobacco etch
                     virus"
     CDS             2304..3020
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    3034..3103
                     /note="computer generated random sequence as a tag of the 
                     HTA6-sGFP fusion transcript"
     regulatory      3104..3319
                     /label=cauliflower mosaic virus 35S poly(A) signal
                     /note="cauliflower mosaic virus 35S poly(A) signal"
                     /regulatory_class="polyA_signal_sequence"
     primer_bind     complement(3341..3357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      3950..4202
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4224..4248
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5548..6174
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6611..7675
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7744..7938
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8282..8422
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8608..9196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9286..10077)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    10502..10526
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10604..10778)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10821..11843)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(11911..12588)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    12779..12800
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12815..12845
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12853..12869
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12877..12893
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"