pcGFP1Fpac vector (V008543)

LOCUS       40924_10566        6754 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway cloning destination vector pcGFP1Fpac, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6754)
  AUTHORS   Dawson SC, House SA.
  TITLE     Imaging and analysis of the microtubule cytoskeleton in giardia
  JOURNAL   Methods Cell Biol. 97, 307-339 (2010)
  PUBMED    20719278
REFERENCE   2  (bases 1 to 6754)
  AUTHORS   Hagen KD, Hirakawa MP, House SA, Schwartz CL, Pham JK, Cipriano MJ, 
            De La Torre MJ, Sek AC, Du G, Forsythe BM, Dawson SC.
  TITLE     Novel structural components of the ventral disc and lateral crest in
            Giardia intestinalis
  JOURNAL   PLoS Negl Trop Dis 5 (12), e1442 (2011)
  PUBMED    22206034
REFERENCE   3  (bases 1 to 6754)
  AUTHORS   Cipriano MJ, Pham JK, Hagen KD, Dawson SC.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2018) Dept of Microbiology and Molecular Genetics,
            University of California, Davis, One Shields Ave, Davis, CA 95616, 
            USA
REFERENCE   4  (bases 1 to 6754)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6754)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Cell Biol. 97, 307-339 (2010)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis"; date: "2011"; volume: "5"; issue: "12"; pages: "e1442"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-2018) Dept of Microbiology and Molecular Genetics, 
            University of California, Davis, One Shields Ave, Davis, CA 95616, 
            USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    893..914
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        929..959
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    967..983
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     991..1007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1025..1043
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    1067..1191
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1216..1246
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1300..1956
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             2301..2603
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2647..2771)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2785..3501
                     /label=EGFP
                     /note="enhanced GFP"
     3'UTR           3514..3638
                     /gene="alpha tubulin"
     gene            3514..3638
                     /gene="alpha tubulin"
                     /label=alpha tubulin
     5'UTR           3717..3919
                     /gene="gamma giardin"
     gene            3717..3919
                     /gene="gamma giardin"
                     /label=gamma giardin
     CDS             3926..4519
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     3'UTR           4529..4646
                     /gene="glutamate dehydrogenase"
     gene            4529..4646
                     /gene="glutamate dehydrogenase"
                     /label=glutamate dehydrogenase
     promoter        complement(4666..4684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4691..4707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4848..5303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5635..5739
                     /label=AmpR promoter
     CDS             5740..6597
                     /label=AmpR
                     /note="beta-lactamase"