pCG181 vector (V008550)

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LOCUS       40924_10531        4433 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway compatible binary vector pCG181, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Generation of marker- and backbone-free transgenic wheat plants via 
            Agrobacterium-mediated transformation based on the pCLEAN dual 
            binary vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Wang G, Jones H, Doherty A, Yu X, Ma Y, Sun Y, Du W, Wang C, Xia L.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2015) Institute of Crop Sciences, Chinese Academy 
            of Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing 
            100081, China
REFERENCE   3  (bases 1 to 4433)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4433)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2015) Institute of Crop Sciences, Chinese Academy of 
            Agricultural Sciences, 12 Zhongguancun Street, Beijing, Beijing 
            100081, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(61..649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(823..1635)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1926..2361
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2508..2532
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    2533..2558
                     /note="spacer sequence (Thole et al., (2007) Plant Physiol 
                     145:1211-1219)"
     misc_feature    2559..2583
                     /note="T-DNA left border No.2 sequence (Thole et al.,
                     (2007) Plant Physiol 145:1211-1219)"
     protein_bind    2599..2723
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2767..3069)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3414..4070)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4124..4154)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(4179..4303)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4351..4375
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    4376..4399
                     /note="right border overdrive sequence (Thole et al.,
                     (2007) Plant Physiol 145:1211-1219)"