pCG1408 vector (V008551)

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LOCUS       40924_10526        9113 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Promoter-probe vector pCG1408, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 5465 to 8413)
  AUTHORS   David M, Bartz SL, Weaver JD, Kelley JL.
  TITLE     Isolation and Characterization of Promoter Sequences from 
            Clavibacter xyli subsp. cynodontis
  JOURNAL   Unpublished
REFERENCE   2  (bases 5465 to 8413)
  AUTHORS   Taylor J, Stearman RS, Uratani BB.
  TITLE     Development of a native plasmid as a cloning vector in Clavibacter 
            xyli subsp. cynodontis
  JOURNAL   Plasmid 29 (3), 241-244 (1993)
  PUBMED    7689234
REFERENCE   3  (bases 1 to 9113)
  AUTHORS   David M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-FEB-1995) Michael David, Crop Genetics, 10150 Old 
            Columbia Rd, Columbia, MD 21046, USA, 21046
REFERENCE   4  (bases 1 to 9113)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Plasmid"; 
            date: "1993"; volume: "29"; issue: "3"; pages: "241-244"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-FEB-1995) Michael David, Crop Genetics, 10150 Old Columbia Rd, 
            Columbia, MD 21046, USA, 21046"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             88..744
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1182..1268
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1360..1387
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      1680..1766
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     regulatory      1684..1726
                     /label=T1
                     /note="T1"
                     /regulatory_class="terminator"
     regulatory      1858..1885
                     /label=T2
                     /note="T2"
                     /regulatory_class="terminator"
     terminator      1858..1885
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2597..2701
                     /label=AmpR promoter
     CDS             2702..3559
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3733..4321
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4507..4647)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(4752..4940)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     rep_origin      5465..8413
                     /label=Clavibacter origin of replication
                     /note="Clavibacter origin of replication"
                     /citation=[1]
                     /citation=[2]
     promoter        complement(8725..8829)
                     /label=AmpR promoter
     terminator      complement(8947..8990)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"