pCFP_Star plasmid pCFP_Star vector (V008552)

LOCUS       V008552                 7255 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008552
VERSION     V008552
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Ectopic Integration Vectors for Generating Fluorescent Promoter
            Fusions in Bacillus subtilis with Minimal Dark Noise
  JOURNAL   PLoS ONE 9 (5), E98360 (2014)
   PUBMED   24874808
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Trauth S, Bischofs IB.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Center for Molecular Biology and BioQuant,
            University of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, BW
            69120, Germany
REFERENCE   3  (bases 1 to 7255)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7255)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2014"; volume: "9"; issue: "5"; pages: "E98360"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2014) Center for Molecular Biology and BioQuant, University
            of Heidelberg, Im Neuenheimer Feld 282, Heidelberg, BW 69120,
            Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        104..208
                     /label="AmpR promoter"
     CDS             209..1066
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1240..1828
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      1829
                     /label="ColE1 origin of DNA replication (+1)"
                     /note="ColE1 origin of DNA replication (+1)"
     misc_feature    complement(2014..2154)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2259..2447)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_recomb     complement(3384..4579)
                     /label="homologous recombination site amyE back"
                     /note="homologous recombination site amyE back"
     CDS             complement(4952..5599)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    5763..5779
                     /label="small cloning site (SCS)"
                     /note="small cloning site (SCS)"
     regulatory      5780..5840
                     /label="Bacillus subtilis gyrA transcriptional terminator"
                     /note="Bacillus subtilis gyrA transcriptional terminator"
                     /regulatory_class="terminator"
     stem_loop       5792..5812
                     /label="Bacillus subtilis gyrA transcriptional terminator"
                     /note="Bacillus subtilis gyrA transcriptional terminator"
     misc_feature    5845..5873
                     /label="LIC site"
                     /note="LIC site"
     CDS             5877..6593
                     /label="mECFP"
                     /note="enhanced CFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_recomb     complement(6618..7255)
                     /label="homologous recombination site amyE front"
                     /note="homologous recombination site amyE front"