pCFPamy vector (V008554)

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LOCUS       V008554                 7265 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008554
VERSION     V008554
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     CDS             complement(661..1377)
                     /label="mECFP"
                     /note="enhanced CFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
     misc_feature    complement(1378..1413)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1566..2213
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2587..3623
                     /label="amyE back"
                     /note="amyE back"
     CDS             4817..5005
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5110..5250
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5436..6024)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6198..7055)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7056..7160)
                     /label="AmpR promoter"