pCFB vector (V008556)

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LOCUS       40924_10471        5291 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCFB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Felts K, Vaillancourt P, Grafsky AJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2000) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   2  (bases 1 to 5291)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2000) Technical Services, Stratagene, 11011 N. Torrey Pines 
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        268..571
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        572..773
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    791
                     /label=transcription initiation start site
                     /note="transcription initiation start site"
     regulatory      993..997
                     /label=SD
                     /note="SD"
                     /regulatory_class="ribosome_binding_site"
     misc_feature    998..1355
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1420..1836
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1846..2219
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2232..2254
                     /label=multiple cloning site
                     /note="multiple cloning site"
     LTR             2338..2931
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      3044
                     /label=ColE1 replication origin
                     /note="ColE1 replication origin"
     misc_feature    3142..3282
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3467..4055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4229..5086)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5087..5191)
                     /label=AmpR promoter