pCEV2 vector (V008560)

LOCUS       40924_10451        8353 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Chloroplast expression vector pCEV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Construction of chloroplast expression vectors for higher plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Chen P-Y., To K-Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2017) Agricultural Biotechnology Research Center, 
            Academia Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 
            11529, Taiwan
REFERENCE   3  (bases 1 to 8353)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2017) Agricultural Biotechnology Research Center, Academia 
            Sinica, No. 128, Academia Road, Section 2, Nankang, Taipei 11529, 
            Taiwan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(26..42)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(50..66)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(74..104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(119..140)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(428..1016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1190..2047)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2048..2152)
                     /label=AmpR promoter
     primer_bind     2626..2642
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2655..4648
                     /note="tomato left targeting region; contains 16S rRNA and
                     trnI"
     rep_origin      4072..4153
                     /note="chloroplast replication origin; oriA"
     regulatory      4655..4902
                     /note="atpI promoter; PatpI"
                     /regulatory_class="promoter"
     misc_feature    4903..4918
                     /label=cloning site
                     /note="cloning site"
     regulatory      4919..5087
                     /note="rps16 terminator; Trps16"
                     /regulatory_class="terminator"
     regulatory      5094..5312
                     /note="rrn promoter; Prrn"
                     /regulatory_class="promoter"
     CDS             5319..6107
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      6117..6338
                     /note="psbA terminator; TpsbA"
                     /regulatory_class="terminator"
     misc_feature    6345..8348
                     /note="tomato right targeting region; contains trnA and 23S
                     rRNA"