pCeMM-NTAP(GS)-Gw vector (V008572)

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LOCUS       40924_10366        8577 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pCeMM-NTAP(GS)-Gw, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Superti-Furga G, Bauch A.
  TITLE     An efficient tandem affinity purification procedure for interaction 
            proteomics in mammalian cells
  JOURNAL   Nat. Methods 3 (12), 1013-1019 (2006)
  PUBMED    17060908
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Bauch A, Superti-Furga G.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2006) Director's Laboratory, Research Center for 
            Molecular Medicine, Lazarettgasse 19/3, Vienna 1090, Austria
REFERENCE   3  (bases 1 to 8577)
  AUTHORS   Burckstummer T, Bennett KL, Preradovic A, Schutze G, Hantschel O, 
            Bauch A, Superti-Furga G.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2006) Director's Laboratory, Research Center for 
            Molecular Medicine, Lazarettgasse 19/3, Vienna 1090, Austria
REFERENCE   4  (bases 1 to 8577)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "12"; pages: "1013-1019"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-NOV-2006) Director's Laboratory, Research Center for Molecular 
            Medicine, Lazarettgasse 19/3, Vienna 1090, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2006) Director's Laboratory, Research Center for Molecular 
            Medicine, Lazarettgasse 19/3, Vienna 1090, Austria"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Dec 14, 2006 this sequence version replaced EF143813.1.
FEATURES             Location/Qualifiers
     source          1..8577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    20..605
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             606..1322
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     LTR             1491..2082
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     CDS             2167..3024
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3198..3786
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4074..4095
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4110..4140
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4148..4164
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4172..4188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4270..4573
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4578..4781
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   4782..4967
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    5020..5377
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             5442..5858
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     promoter        6205..6223
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6653..6673
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             6680..6793
                     /codon_start=1
                     /label=SBP
                     /note="streptavidin-binding peptide"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     protein_bind    6869..6993
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        7018..7048
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             7102..7758
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             8103..8405
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(8449..8573)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"