pCE70 vector (V008575)

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LOCUS       V008575                 6920 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008575
VERSION     V008575
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6920)
  AUTHORS   Merighi M, Ellermeier CD, Slauch JM, Gunn JS.
  TITLE     Resolvase-IVET Analysis of the Salmonella enterica sv. typhimurium
            PhoP and PmrA regulons in BALB/c mice
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6920)
  AUTHORS   Smith JN, Ellermeier CD, Dyszel J, Altier C, Young GM, Lawhon SD,
            Adams LG, Konjufca VK, Curtiss R III, Slauch JM, Ahmer BMM.
  TITLE     Recording of SdiA activity during transit of Salmonella through
            animals
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6920)
  AUTHORS   Ellermeier CD, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2005) Microbiology, University of Illinois
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801,
            USA
REFERENCE   4  (bases 1 to 6920)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6920)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (23-MAY-2005) Microbiology, University of Illinois Urbana-Champaign,
            601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(40..73)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     regulatory      82..89
                     /gene="tnpR"
                     /regulatory_class="ribosome_binding_site"
     CDS             99..647
                     /gene="tnpR"
                     /label="Transposon gamma-delta resolvase"
                     /note="Transposon gamma-delta resolvase from Escherichia
                     coli (strain K12). Accession#: P03012"
     CDS             675..3746
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3801..5051
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      5260..5289
                     /note="histidine bi-directional rho-independent terminator
                     from Salmonella typhimurium"
                     /regulatory_class="terminator"
     CDS             complement(5540..6352)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6522..6910
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"