pELE-1 vector (V007581)

MSDS

Basic Vector Information

Vector Name:: pELE-1

Antibiotic Resistance:: Ampicillin

Length:: 3168 bp

Type:: MoClo end-linker vector

Replication origin:: ori

Source/Author:: Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.

pELE-1 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pELE-1 vector Sequence

Copy Sequence

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LOCUS       40924_17324        3168 bp DNA     circular SYN 17-DEC-2018
DEFINITION  MoClo end-linker vector pELE-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     A modular cloning system for standardized assembly of multigene 
            constructs
  JOURNAL   PLoS ONE 6 (2), E16765 (2011)
  PUBMED    21364738
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, 
            Halle/Saale 06120, Germany
REFERENCE   3  (bases 1 to 3168)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2011"; volume: "6"; issue: "2"; pages: "E16765"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, Halle/Saale 06120,
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..36)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        510..614
                     /label=AmpR promoter
     CDS             615..1472
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1646..2234
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2522..2543
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2558..2588
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2596..2612
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2620..2636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2698..2701
                     /note="fusion site; other site"
     misc_feature    2722..2725
                     /note="fusion site; other site"

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