Basic Vector Information
- Vector Name:
- pELB-1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4945 bp
- Type:
- MoClo end-linker vector
- Replication origin:
- oriV
- Source/Author:
- Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
pELB-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pELB-1 vector Sequence
LOCUS 40924_17319 4945 bp DNA circular SYN 17-DEC-2018
DEFINITION MoClo end-linker vector pELB-1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4945)
AUTHORS Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
TITLE A modular cloning system for standardized assembly of multigene
constructs
JOURNAL PLoS ONE 6 (2), E16765 (2011)
PUBMED 21364738
REFERENCE 2 (bases 1 to 4945)
AUTHORS Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S.
TITLE Direct Submission
JOURNAL Submitted (20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22,
Halle/Saale 06120, Germany
REFERENCE 3 (bases 1 to 4945)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4945)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2011"; volume: "6"; issue: "2"; pages: "E16765"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-DEC-2010) Icon Genetics GmbH, Weinbergweg 22, Halle/Saale 06120,
Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4945
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 22..46
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS complement(205..1350)
/label=trfA
/note="trans-acting replication protein that binds to and
activates oriV"
rep_origin complement(1686..2274)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2448..3305)
/label=AmpR
/note="beta-lactamase"
promoter complement(3306..3410)
/label=AmpR promoter
rep_origin complement(3427..4136)
/direction=LEFT
/label=oriV
/note="incP origin of replication"
misc_feature 4220..4244
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
misc_feature 4333..4336
/note="fusion site; other site"
protein_bind 4399..4420
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4435..4465
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4473..4489
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4497..4513
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_feature complement(4526..4582)
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(4583..4599)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 4929..4932
/note="fusion site; other site"
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