Basic Vector Information
- Vector Name:
- pEGSH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4620 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhang C-F., Grafsky AJ.
- Promoter:
- HSV TK
pEGSH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGSH vector Sequence
LOCUS 40924_17289 4620 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEGSH, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4620)
AUTHORS Zhang C-F., Grafsky AJ.
TITLE Direct Submission
JOURNAL Submitted (04-NOV-1998) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 2 (bases 1 to 4620)
AUTHORS Zhang C-F., Grafsky AJ.
TITLE Direct Submission
JOURNAL Submitted (03-MAR-1999) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 3 (bases 1 to 4620)
AUTHORS Zhang C-F., Grafsky AJ.
TITLE Direct Submission
JOURNAL Submitted (06-JUN-2018) Technical Services, Stratagene, 11011 N.
Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE 4 (bases 1 to 4620)
TITLE Direct Submission
REFERENCE 5 (bases 1 to 4620)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(04-NOV-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-MAR-1999) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
(06-JUN-2018) Technical Services, Stratagene, 11011 N. Torrey Pines
Rd., La Jolla, CA 92037, USA"
COMMENT SGRef: number: 4; type: "Journal Article"
COMMENT On Jun 6, 2018 this sequence version replaced AF104248.2.
FEATURES Location/Qualifiers
source 1..4620
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 293..438
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
CDS 481..1515
/codon_start=1
/label=HygR
/note="hygromycin B phosphotransferase"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
polyA_signal 1559..1607
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin complement(1933..2521)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2695..3552)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3560..3664)
/label=AmpR promoter
rep_origin 3691..4146
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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