Basic Vector Information
- Vector Name:
- pEGPF-N2-NRSE4x
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5121 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Koch JC, Barski E, Lingor P, Bahr M, Michel U.
- Promoter:
- CMV
pEGPF-N2-NRSE4x vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGPF-N2-NRSE4x vector Sequence
LOCUS 40924_17284 5121 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEGPF-N2-NRSE4x, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5121)
AUTHORS Koch JC, Barski E, Lingor P, Bahr M, Michel U.
TITLE Plasmids containing NRSE/RE1 sites enhance neurite outgrowth of
retinal ganglion cells via sequestration of REST independent of NRSE
dsRNA expression
JOURNAL FEBS J. 278 (18), 3472-3483 (2011)
PUBMED 21790997
REFERENCE 2 (bases 1 to 5121)
AUTHORS Koch JC, Michel U.
TITLE Direct Submission
JOURNAL Submitted (18-OCT-2010) Neurology, University Medicine Goettingen,
Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany
REFERENCE 3 (bases 1 to 5121)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5121)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "FEBS J.";
date: "2011"; volume: "278"; issue: "18"; pages: "3472-3483"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-OCT-2010) Neurology, University Medicine Goettingen,
Robert-Koch-Str. 40, Goettingen, Niedersachsen 37075, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5121
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal complement(665..786)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 900..1012
/note="NRSE sequence; 4 fold"
CDS 1067..1783
/label=EGFP
/note="enhanced GFP"
polyA_signal 1909..2030
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2037..2492)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2519..2623
/label=AmpR promoter
promoter 2625..2982
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3017..3808
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 4043..4090
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4419..5007
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.