pEGFP-Pax9 vector (V007591)

Basic Vector Information

Vector Name:
pEGFP-Pax9
Antibiotic Resistance:
Kanamycin
Length:
5738 bp
Type:
Expression vector
Replication origin:
ori
Source/Author:
Wang Z, Lan J, Chen Z, Fan M.

pEGFP-Pax9 vector Vector Map

pEGFP-Pax95738 bp60012001800240030003600420048005400CMV enhancerCMV promoterEGFPPaired box protein Pax-9SV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

pEGFP-Pax9 vector Sequence

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Download GeneBank File(.gb)

LOCUS       V007591                 5738 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007591
VERSION     V007591
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     The Construction and Expression of Green Fluorescent Protein (GFP)-
            Paired-box Gene 9 (Pax9) Fused Protein Eukaryotic Cell Expression
            Plasmid and Subcellular Location of Pax9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2005) School of Stomatology, Wuhan University,
            Luoyu Road 65#, Wuhan, Hubei 430079, China
REFERENCE   3  (bases 1 to 5738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-APR-2005) School of Stomatology, Wuhan University, Luoyu Road
            65#, Wuhan, Hubei 430079, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1366..2391
                     /gene="Pax9"
                     /label="Paired box protein Pax-9"
                     /note="Paired box protein Pax-9 from Mus musculus.
                     Accession#: P47242"
     polyA_signal    2526..2647
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2654..3109)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3136..3240
                     /label="AmpR promoter"
     promoter        3242..3599
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3634..4425
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4660..4707
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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