pEGFP-Pax9 vector (V007591)

MSDS

Basic Vector Information

Vector Name:: pEGFP-Pax9

Antibiotic Resistance:: Kanamycin

Length:: 5738 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Wang Z, Lan J, Chen Z, Fan M.

pEGFP-Pax9 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pEGFP-Pax9 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V007591                 5738 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007591
VERSION     V007591
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     The Construction and Expression of Green Fluorescent Protein (GFP)-
            Paired-box Gene 9 (Pax9) Fused Protein Eukaryotic Cell Expression
            Plasmid and Subcellular Location of Pax9
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Wang Z, Lan J, Chen Z, Fan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-APR-2005) School of Stomatology, Wuhan University,
            Luoyu Road 65#, Wuhan, Hubei 430079, China
REFERENCE   3  (bases 1 to 5738)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5738)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-APR-2005) School of Stomatology, Wuhan University, Luoyu Road
            65#, Wuhan, Hubei 430079, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1366..2391
                     /gene="Pax9"
                     /label="Paired box protein Pax-9"
                     /note="Paired box protein Pax-9 from Mus musculus.
                     Accession#: P47242"
     polyA_signal    2526..2647
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2654..3109)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3136..3240
                     /label="AmpR promoter"
     promoter        3242..3599
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3634..4425
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4660..4707
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5036..5624
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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