Basic Vector Information
- Vector Name:
- pEGFP-neo4
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6125 bp
- Type:
- Transformation vector
- Replication origin:
- ori
- Source/Author:
- Kataoka K, Schoeberl UE, Mochizuki K.
pEGFP-neo4 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEGFP-neo4 vector Sequence
LOCUS 40924_17209 6125 bp DNA circular SYN 17-DEC-2018
DEFINITION Transformation vector pEGFP-neo4 DNA, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6125)
AUTHORS Kataoka K, Schoeberl UE, Mochizuki K.
TITLE Modules for C-terminal epitope tagging of Tetrahymena genes
JOURNAL J. Microbiol. Methods 82 (3), 342-346 (2010)
PUBMED 20624430
REFERENCE 2 (bases 1 to 6125)
AUTHORS Kataoka K, Mochizuki K.
TITLE Direct Submission
JOURNAL Submitted (29-JUN-2010) Contact:Kazufumi Mochizuki Institute of
Molecular Biotechnology of the Austrian Academy of Sciences (IMBA);
Dr. Bohr-Gasse 3, Vienna A-1030, Austria
REFERENCE 3 (bases 1 to 6125)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6125)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Microbiol. Methods"; date: "2010"; volume: "82"; issue: "3"; pages:
"342-346"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(29-JUN-2010) Contact:Kazufumi Mochizuki Institute of Molecular
Biotechnology of the Austrian Academy of Sciences (IMBA); Dr.
Bohr-Gasse 3, Vienna A-1030, Austria"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6125
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 21..125
/label=AmpR promoter
CDS 126..983
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1157..1745
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 2033..2054
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2069..2099
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2107..2123
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2131..2147
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 2168..2186
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 2247..2963
/codon_start=1
/label=EGFP
/note="enhanced GFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 2970..3368
/label=BTU1 3' flanking
/note="BTU1 3' flanking"
CDS 4280..5071
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5440..5456)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(5482..5500)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5507..5523)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 5665..6120
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
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