pEGFP-hnRNPC1 vector (V007599)

Basic Vector Information

Vector Name:
pEGFP-hnRNPC1
Antibiotic Resistance:
Kanamycin
Length:
5631 bp
Type:
Mammalian expression vector
Replication origin:
ori
Source/Author:
De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
Promoter:
CMV

pEGFP-hnRNPC1 vector Vector Map

pEGFP-hnRNPC15631 bp60012001800240030003600420048005400CMV enhancerCMV promoterEGFPHeterogeneous nuclear ribonucleoprotein CSV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRHSV TK poly(A) signalori

pEGFP-hnRNPC1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V007599                 5631 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007599
VERSION     V007599
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5631)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1381..2286
                     /gene="HNRNPC"
                     /label="Heterogeneous nuclear ribonucleoprotein C"
                     /note="Heterogeneous nuclear ribonucleoprotein C from Pongo
                     abelii. Accession#: Q5RA82"
     polyA_signal    2419..2540
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2547..3002)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3029..3133
                     /label="AmpR promoter"
     promoter        3135..3492
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3527..4318
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4553..4600
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4929..5517
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"

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