Basic Vector Information
- Vector Name:
- pEGFP-hnRNPC1
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5631 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- CMV
pEGFP-hnRNPC1 vector Map
pEGFP-hnRNPC1 vector Sequence
LOCUS V007599 5631 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V007599 VERSION V007599 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 5631) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 5631) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 5631) TITLE Direct Submission REFERENCE 4 (bases 1 to 5631) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5631 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 61..364 /label="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label="CMV promoter" /note="human cytomegalovirus (CMV) immediate early promoter" CDS 613..1329 /label="EGFP" /note="enhanced GFP" CDS 1381..2286 /gene="HNRNPC" /label="Heterogeneous nuclear ribonucleoprotein C" /note="Heterogeneous nuclear ribonucleoprotein C from Pongo abelii. Accession#: Q5RA82" polyA_signal 2419..2540 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" rep_origin complement(2547..3002) /direction=LEFT /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3029..3133 /label="AmpR promoter" promoter 3135..3492 /label="SV40 promoter" /note="SV40 enhancer and early promoter" CDS 3527..4318 /label="NeoR/KanR" /note="aminoglycoside phosphotransferase" polyA_signal 4553..4600 /label="HSV TK poly(A) signal" /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4929..5517 /direction=RIGHT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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