pEG vector (V007603)

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LOCUS       V007603                 4151 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007603
VERSION     V007603
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Zhou Y, Zhang L, Shah N, Palmer R Jr.
  TITLE     Cell-surface glycoside hydrolases of Streptococcus gordonii promote
            growth in saliva
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Yang J, Cisar JO.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2015) Laboratory of Cell and Developmental
            Biology, National Institute of Health, 9000 Rockville Pike,
            Bethesda, MD 20854, USA
REFERENCE   3  (bases 1 to 4151)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-OCT-2015) Laboratory of Cell and Developmental Biology, National
            Institute of Health, 9000 Rockville Pike, Bethesda, MD 20854, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      402..599
                     /note="consititutive promoter located upstream of
                     Streptococcus gordonii rpsJ gene"
                     /regulatory_class="promoter"
     CDS             600..1313
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    1320..1376
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1389..1405)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1413..1429)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1437..1467)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1482..1503)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1791..2379)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             2951..3685
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     promoter        complement(3952..4056)
                     /label="AmpR promoter"