Basic Vector Information
- Vector Name:
- pEG
- Length:
- 4151 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Zhou Y, Zhang L, Shah N, Palmer R Jr.
pEG vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEG vector Sequence
LOCUS V007603 4151 bp DNA circular SYN 17-DEC-2018
DEFINITION Exported.
ACCESSION V007603
VERSION V007603
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 4151)
AUTHORS Zhou Y, Zhang L, Shah N, Palmer R Jr.
TITLE Cell-surface glycoside hydrolases of Streptococcus gordonii promote
growth in saliva
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4151)
AUTHORS Yang J, Cisar JO.
TITLE Direct Submission
JOURNAL Submitted (26-OCT-2015) Laboratory of Cell and Developmental
Biology, National Institute of Health, 9000 Rockville Pike,
Bethesda, MD 20854, USA
REFERENCE 3 (bases 1 to 4151)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4151)
AUTHORS .
TITLE Direct Submission
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-OCT-2015) Laboratory of Cell and Developmental Biology, National
Institute of Health, 9000 Rockville Pike, Bethesda, MD 20854, USA"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4151
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
regulatory 402..599
/note="consititutive promoter located upstream of
Streptococcus gordonii rpsJ gene"
/regulatory_class="promoter"
CDS 600..1313
/label="yeGFP"
/note="yeast-enhanced green fluorescent protein"
misc_feature 1320..1376
/label="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(1389..1405)
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1413..1429)
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1437..1467)
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind complement(1482..1503)
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1791..2379)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 2951..3685
/gene="ermBP"
/label="rRNA adenine N-6-methyltransferase"
/note="rRNA adenine N-6-methyltransferase from Enterococcus
faecalis. Accession#: P0A4D5"
promoter complement(3952..4056)
/label="AmpR promoter"
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