Basic Vector Information
- Vector Name:
- pEG(KT)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9267 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Pierce BD, Wendland B.
- Promoter:
- URA3
pEG(KT) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEG(KT) vector Sequence
LOCUS 40924_17104 9267 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector pEG(KT), complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9267)
AUTHORS Pierce BD, Wendland B.
TITLE Sequence of the yeast protein expression plasmid pEG(KT)
JOURNAL Yeast 26 (6), 349-353 (2009)
PUBMED 19350530
REFERENCE 2 (bases 1 to 9267)
AUTHORS Pierce BD, Wendland B.
TITLE Direct Submission
JOURNAL Submitted (03-DEC-2008) Biology, The Johns Hopkins University, 3400
N. Charles St., Mudd Hall, Baltimore, MD 21218, USA
REFERENCE 3 (bases 1 to 9267)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9267)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
date: "2009"; volume: "26"; issue: "6"; pages: "349-353"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(03-DEC-2008) Biology, The Johns Hopkins University, 3400 N. Charles
St., Mudd Hall, Baltimore, MD 21218, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9267
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 22..39
/label=thrombin site
/note="thrombin recognition and cleavage site"
rep_origin 327..1309
/label=2u ori
/note="yeast 2u plasmid origin of replication"
CDS 1676..2767
/label=LEU2
/note="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
primer_bind complement(3021..3037)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3477..3990
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4735..4839
/label=AmpR promoter
CDS 4840..5697
/label=AmpR
/note="beta-lactamase"
rep_origin 5871..6459
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6746..6767
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6782..6812
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6820..6836
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6844..6860
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 6880..7091
/label=URA3 promoter
CDS 7092..7892
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
protein_bind complement(8152..8269)
/label=UAS
/note="upstream activating sequence mediating
Gal4-dependent induction"
regulatory 8344..8589
/label=CYC1
/note="CYC1"
/regulatory_class="promoter"
CDS 8608..9261
/label=GST
/note="glutathione S-transferase from Schistosoma
japonicum"
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