pEG(KT) vector (V007604)

LOCUS       40924_17104        9267 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEG(KT), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Pierce BD, Wendland B.
  TITLE     Sequence of the yeast protein expression plasmid pEG(KT)
  JOURNAL   Yeast 26 (6), 349-353 (2009)
  PUBMED    19350530
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Pierce BD, Wendland B.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2008) Biology, The Johns Hopkins University, 3400 
            N. Charles St., Mudd Hall, Baltimore, MD 21218, USA
REFERENCE   3  (bases 1 to 9267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2009"; volume: "26"; issue: "6"; pages: "349-353"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-DEC-2008) Biology, The Johns Hopkins University, 3400 N. Charles
            St., Mudd Hall, Baltimore, MD 21218, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..39
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     rep_origin      327..1309
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             1676..2767
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     primer_bind     complement(3021..3037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3477..3990
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4735..4839
                     /label=AmpR promoter
     CDS             4840..5697
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5871..6459
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6746..6767
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6782..6812
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6820..6836
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6844..6860
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6880..7091
                     /label=URA3 promoter
     CDS             7092..7892
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     protein_bind    complement(8152..8269)
                     /label=UAS
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     regulatory      8344..8589
                     /label=CYC1
                     /note="CYC1"
                     /regulatory_class="promoter"
     CDS             8608..9261
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"