Basic Vector Information
- Vector Name:
- pEFBOS-IRESGFPNeo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8103 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Lim SM, Stanley EG, Elefanty AG.
- Promoter:
- EF-1α
pEFBOS-IRESGFPNeo vector Map
pEFBOS-IRESGFPNeo vector Sequence
LOCUS 40924_17094 8103 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector pEFBOS-IRESGFPNeo, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8103) AUTHORS Lim SM, Stanley EG, Elefanty AG. TITLE pEFBOS-IRESGFPNeo, a derivative of pEFBOS containing an IRESGFPNeo cassette JOURNAL Unpublished REFERENCE 2 (bases 1 to 8103) AUTHORS Lim SM, Stanley EG, Elefanty AG. TITLE Direct Submission JOURNAL Submitted (27-FEB-2007) Monash Immunology and Stem Cell Laboratories, Monash University, West Ring Road, Clayton, Vic 3800, Australia REFERENCE 3 (bases 1 to 8103) TITLE Direct Submission REFERENCE 4 (bases 1 to 8103) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-FEB-2007) Monash Immunology and Stem Cell Laboratories, Monash University, West Ring Road, Clayton, Vic 3800, Australia" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..8103 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 107..128 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter complement(333..543) /label=SV40 promoter /note="SV40 early promoter" promoter 562..1743 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" misc_feature 1826..2412 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 2425..3135 /codon_start=1 /label=mgfp5 /note="GFP with folding enhancement mutations" /translation="SKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLKF ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN FKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF VTAAGITHGMDELYK" CDS 3145..3933 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL ATRDIAEELCGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" 3'UTR 4523..5223 /label=bovine growth hormone 3' UTR /note="bovine growth hormone 3' UTR" primer_bind complement(5232..5248) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 5461..5916 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 6198..6302 /label=AmpR promoter CDS 6303..7160 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7334..7922 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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