Basic Vector Information
- Vector Name:
- pEF6-hXIAP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7589 bp
- Type:
- Mammalian expression vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- EF-1α
pEF6-hXIAP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pEF6-hXIAP vector Sequence
LOCUS V007615 7589 bp DNA circular SYN 17-DEC-2018 DEFINITION Exported. ACCESSION V007615 VERSION V007615 KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct . REFERENCE 1 (bases 1 to 7589) AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished REFERENCE 2 (bases 1 to 7589) AUTHORS De Schamphelaire W. TITLE Direct Submission JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM REFERENCE 3 (bases 1 to 7589) TITLE Direct Submission REFERENCE 4 (bases 1 to 7589) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM" SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..7589 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 351..1529 /label="EF-1-alpha promoter" /note="strong constitutive promoter for human elongation factor EF-1-alpha" promoter 1546..1564 /label="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" CDS 1661..1690 /label="Myc" /note="Myc (human c-Myc proto-oncogene) epitope tag" CDS 1700..1729 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" CDS 1739..1768 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" CDS 1778..1807 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" CDS 1817..1846 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" CDS 1880..1909 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label="Myc" /translation="EQKLISEEDL" CDS 1934..3424 /gene="XIAP" /label="E3 ubiquitin-protein ligase XIAP" /note="E3 ubiquitin-protein ligase XIAP from Homo sapiens. Accession#: P98170" CDS 3471..3500 /label="Myc" /note="Myc (human c-Myc proto-oncogene) epitope tag" CDS 3516..3533 /label="6xHis" /note="6xHis affinity tag" polyA_signal 3562..3786 /label="bGH poly(A) signal" /note="bovine growth hormone polyadenylation signal" rep_origin 3832..4260 /label="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 4274..4603 /label="SV40 promoter" /note="SV40 enhancer and early promoter" promoter 4651..4698 /label="EM7 promoter" /note="synthetic bacterial promoter" CDS 4717..5112 /label="BSD" /note="blasticidin S deaminase" polyA_signal 5273..5406 /label="SV40 poly(A) signal" /note="SV40 polyadenylation signal" primer_bind complement(5443..5459) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" protein_bind complement(5467..5483) /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(5491..5521) /label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind complement(5536..5557) /label="CAP binding site" /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(5834..6422) /direction=LEFT /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6596..7453) /label="AmpR" /note="beta-lactamase" promoter complement(7454..7558) /label="AmpR promoter"
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