pEF-F13F14-tdTomato-dpuro vector (V007639)

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LOCUS       40924_16855        6084 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pEF-F13F14-tdTomato-dpuro, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Baser B, Spehr J, Bussow K, van den Heuvel J.
  TITLE     A method for specifically targeting two independent genomic 
            integration sites for co-expression of genes in CHO cells
  JOURNAL   Methods (2015) In press
  PUBMED    26658354
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Baser B, Spehr J, Buessow K, van den Heuvel J.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) Structure and Function of Proteins, 
            Recombinant Protein Expression, Helmholtz Centre for Infection 
            Research, Inhoffenstrasse 7, Braunschweig, Lower Saxony 38124, 
            Germany
REFERENCE   3  (bases 1 to 6084)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUL-2015) Structure and Function of Proteins, Recombinant 
            Protein Expression, Helmholtz Centre for Infection Research, 
            Inhoffenstrasse 7, Braunschweig, Lower Saxony 38124, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        111..1300
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     misc_recomb     1322..1369
                     /note="FRT13; synthetic variant 13 of the yeast
                     flp-recombinase target (FRT)"
     CDS             1388..2815
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     CDS             2816..2833
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    complement(2851..2985)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     3098..3145
                     /note="FRT14; synthetic variant 14 of the yeast
                     flp-recombinase target (FRT)"
     CDS             3165..3758
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     promoter        complement(3805..3823)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3849..4073
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4332..4920)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5094..5951)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5952..6056)
                     /label=AmpR promoter