pEBM113 vector (V007676)

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LOCUS       V007676                 9485 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007676
VERSION     V007676
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Mearls EB, Olson DG, Herring CD, Lynd LR.
  TITLE     Development of a regulatable plasmid-based gene expression system
            for Clostridium thermocellum
  JOURNAL   Appl. Microbiol. Biotechnol. 99 (18), 7589-7599 (2015)
   PUBMED   25994254
REFERENCE   2  (bases 1 to 9485)
  AUTHORS   Argyros A, Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 9485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Microbiol. Biotechnol."; date: "2015"; volume: "99"; issue: "18";
            pages: "7589-7599"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      57..304
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(552..1140)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1314..2171)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(2270..3070)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3071..3291)
                     /label="URA3 promoter"
     misc_feature    3319..3822
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     CDS             complement(4054..5055)
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      5541..6188
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            5541..6188
                     /gene="gapD"
                     /label="gapD"
     CDS             6189..6836
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     regulatory      7417..7456
                     /regulatory_class="terminator"
     regulatory      7464..7502
                     /gene="celC"
                     /regulatory_class="promoter"
     gene            7464..7502
                     /gene="celC"
                     /label="celC"
     CDS             7626..8663
                     /codon_start=1
                     /gene="glyR3"
                     /product="GlyR3"
                     /label="glyR3"
                     /note="repressor"
                     /protein_id="AGT95728.1"
                     /translation="MTSEEIAKLCGVSRATVSRVINNSPNVKEETRQKILAVIKEKNYV
                     PIAPARRLAGIDSNIIGLFVLDIDISESKSRVSESTYFSRLINLIIDQANNFGFQVLVS
                     IITSQKQLSEIRNLFMSRTIFSGIFIGAFNDEIQLDDDIIMQHPTIIIDRQSERMVKKP
                     NRLVVNLDNFEGAYNATQFLIKLGHTRIGHISGDLRKLSGIERYEGYKKALEDAGLGFD
                     KNLVREGNFLDDSGYRLAREILKENVTAIFCANDVMAISAIKAIKETGLSVPDDISVIG
                     FDNTAIGNYIMPALTTVNAPLEHIAEACIESLKYFCEHKHFKQKEIRVKTDLIIRDSTK
                     RALEF"
     gene            7626..8663
                     /gene="glyR3"
                     /label="glyR3"
     CDS             8676..9485
                     /codon_start=1
                     /gene="spo0A"
                     /product="Spo0A"
                     /label="spo0A"
                     /note="sporulation response regulator A"
                     /protein_id="AGT95729.1"
                     /translation="MTSNKIRVVIADDNREFGDILYEYLNNQEDIEVVGVARDGIEAYE
                     LIVEKLPDIAILDIIMPHLDGLGVLEKIGATAISKRPLFIILSAVGQDKITQRALALGA
                     EYYVVKPFDMEVLISRIRQLKNVNQPNVIRQDGLSGEVKSSYHPPQPKNLEAEVTNIMH
                     EIGVPAHIKGYQYLRDAIIMVVKDLDVINSITKQLYPTIAKEYNTTPSRVERAIRHAIE
                     VAWSRGQIDTIDSLFGYTINVGKGKPTNSEFIAMVADKLRLEMKMAT"
     gene            8676..9485
                     /gene="spo0A"
                     /label="spo0A"