pEBM100 vector (V007682)

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LOCUS       V007682                12613 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007682
VERSION     V007682
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12613)
  AUTHORS   Lynd LR.
  TITLE     Identification of sporulation histidine kinases in C. thermocellum
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12613)
  AUTHORS   Mearls EB.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2013) Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 12613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2013) Engineering, Dartmouth College, 14 Engineering Dr,
            Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..2139
                     /codon_start=1
                     /product="histidine kinase"
                     /label="histidine kinase"
                     /note="from Clostridium thermocellum Clo1313_1973"
                     /protein_id="AGO62061.1"
                     /translation="MKKRRTIGFLVNDIDVSYSNLVYLMTKKAAEKYDCNLIVYEGRSF
                     NNPSMADSQHQISYSFVDNTRLDGIIITSATIACFISDEEFVEFCKRYEGIPLVSLGIV
                     VPHATSILVDNKPGMKSQVEHLIKDHGFKKIVYISGPPTNTDSVERLEAYCEALRENDI
                     EVDDGLIFYGDFMAQSGYDIIKEIIKNGIEYDAVVCANDEMAFGAIKGIRDLKDAYNLD
                     LTQKGVICGFDDSITSRLVKPSLTTVRQPIEEMCFYAVEAILKKIEGEKVEDVVKLPSV
                     LVKRESCGCKNAAEVDFVSDSSLRLVPGQRIHENLQTYSLDELFDCITRTLKLCSIRSC
                     FIFKYCGGTLLYEVDSIFRDDFRLPEDSELIYAYYNDERITIEDSIKYIKTRCIIPDRF
                     FNEDRRFTYLVNPLFFYDEHFGYVCFEVANDDVLNFEPIRGQISNTLNGALMLMERAKM
                     EESLRETERLASLGQLIGGISHNLMTPIMSISGACFGLEDLVHEYMASIEDPSVTVQDH
                     KEIARDMQEWINKLKEYNSYMSNVIATVKSQAVQLNSDAINEFTIEELVDRVKFIKNNN
                     PQIRKAVLNCKIDIDGKTAIRGDISNLAQIIENLLINAVQSYEGMDNASNIVDLHILKE
                     ENMVKCIVRDYGKGIPNEIRDRLFRRMVTTKGKNGTGLSLLLSYSTIKGKFGGELWVES
                     KENEGSAFYVTIPLTDRE"
     terminator      2189..2436
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2684..3272)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3359..3937)
                     /codon_start=1
                     /product="tdk"
                     /label="tdk"
                     /protein_id="AGO62062.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     regulatory      3938..4558
                     /label="cbp promoter"
                     /note="cbp promoter"
                     /regulatory_class="promoter"
     CDS             complement(4637..5638)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             complement(6199..7056)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(7155..7955)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(7956..8176)
                     /label="URA3 promoter"
     misc_feature    8204..8707
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_recomb     8868..9809
     misc_recomb     9810..10673
     regulatory      10691..11338
                     /gene="gapD"
                     /regulatory_class="promoter"
     gene            10691..11338
                     /gene="gapD"
                     /label="gapD"
     CDS             11339..11986
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             12007..12552
                     /codon_start=1
                     /gene="hpt"
                     /product="hpt"
                     /label="hpt"
                     /note="hypoxanthine phosphoribosyltransferase"
                     /protein_id="AGO62065.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            12007..12552
                     /gene="hpt"
                     /label="hpt"