pdvec0 vector (V007708)

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LOCUS       40924_16385        4436 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Phage display vector pdvec0 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Makino Y, Ishida S, Itoh N.
  TITLE     Phage display vector pdvec0
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Makino Y, Itoh N.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JUL-2018) Contact:Yoshihide Makino Toyama Prefectural 
            Univerisity, Department of Biotechnology, Faculty of Engineering; 
            5180 Kurokawa, Imizu-city, Toyama 939-0398, Japan URL 
            :http://www.pu-toyama.ac.jp/
REFERENCE   3  (bases 1 to 4436)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4436)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JUL-2018) Contact:Yoshihide Makino Toyama Prefectural 
            Univerisity, Department of Biotechnology, Faculty of Engineering"; 
            volume: " 5180 Kurokawa, Imizu-city, Toyama 939-0398, Japan URL 
            :http"; pages: "//www.pu-toyama.ac.jp"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    217..270
                     /gene="III"
                     /note="gene III signal peptide of Mq3KO7. This region
                     introduces the express of a protein structural gene 
                     inserted in the downstream of this region."
     misc_feature    271..336
                     /gene="III"
                     /note="A protein structural gene can be inserted in this
                     region. The inserted gene is expressed as a fusion peptide 
                     of (geneIII signal peptide of M13KO7)-(polypeptide from 
                     inserted sequence)-(geneIII mature peptide M13KO7)."
     CDS             334..1554
                     /label=M13 gene III
                     /note="pIII"
     primer_bind     complement(1565..1581)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1794..2249
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2531..2635
                     /label=AmpR promoter
     CDS             2636..3493
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3667..4255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"