pDUAL2-HFF81 vector (V007742)

LOCUS       40924_16195        8400 bp DNA     circular SYN 17-DEC-2018
DEFINITION  S. pombe expression vector pDUAL2-HFF81 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8400)
  AUTHORS   Matsuyama A, Shirai A, Yashiroda Y, Kamata A, Horinouchi S, Yoshida 
            M.
  TITLE     pDUAL, a multipurpose, multicopy vector capable of chromosomal 
            integration in fission yeast
  JOURNAL   Yeast 21 (15), 1289-1305 (2004)
  PUBMED    15546162
REFERENCE   2  (bases 1 to 8400)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast multicopy and integration vector pDUAL2-HFF81
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 8400)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Akihisa Matsuyama, RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan 
            (E-mail:akihisa@riken.jp, URL:http://cgl.riken.go.jp/, 
            Tel:81-48-462-1335, Fax:81-48-462-1336)
REFERENCE   4  (bases 1 to 8400)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 8400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004"; volume: "21"; issue: "15"; pages: "1289-1305"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Akihisa Matsuyama, RIKEN, Chemical Genetics 
            Laboratory"; volume: " Hirosawa 2-1, Wako, Saitama 351-0198, Japan 
            (E-mail:akihisa@riken.jp, URL:http://cgl.riken.go.jp/, 
            Tel:81-48-462-1335, Fax"; pages: "81-48-462-1336"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..8400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     rep_origin      975..1758
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(2206..2222)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2435..2890
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3172..3276
                     /label=AmpR promoter
     CDS             3277..4134
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4308..4896
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5184..5205
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5220..5250
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5258..5274
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5282..5298
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(5317..5746)
                     /gene="ura4"
                     /regulatory_class="terminator"
     CDS             complement(5750..6541)
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      complement(6542..7068)
                     /gene="ura4"
                     /regulatory_class="promoter"
     promoter        7117..8266
                     /label=nmt1 P41 promoter
                     /note="mutant nmt1 promoter from Schizosaccharomyces pombe,
                     conferring medium strength thiamine-repressible expression"
     CDS             8295..8312
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             8316..8339
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             8346..8369
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"