pDREA vector (V007930)

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LOCUS       V007930                10070 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007930
VERSION     V007930
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Wojcik EA, Brzostek A, Bacolla A, Mackiewicz P, Vasquez KM,
            Korycka-Machala M, Jaworski A, Dziadek J.
  TITLE     Direct and inverted repeats elicit genetic instability by both
            exploiting and eluding DNA double-strand break repair systems in
            mycobacteria
  JOURNAL   PLoS ONE 7 (12), E51064 (2012)
   PUBMED   23251422
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Dziadek J, Wojcik EA.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2012) Pharmacology and Toxicology, University of
            Texas, 1400 Barbara Jordan Blvd., Austin, TX 78759, USA
REFERENCE   3  (bases 1 to 10070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2012"; volume: "7"; issue: "12"; pages: "E51064"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-OCT-2012) Pharmacology and Toxicology, University of Texas, 1400
            Barbara Jordan Blvd., Austin, TX 78759, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1267..1855
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     rep_origin      complement(2028..3927)
                     /direction=LEFT
                     /label="mycobacterial OriM region from pAL5000"
                     /note="mycobacterial OriM region from pAL5000"
     CDS             4201..7245
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             complement(7513..8460)
                     /gene="hyg"
                     /label="Hygromycin-B 7''-O-kinase"
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces
                     hygroscopicus. Accession#: P09979"
     CDS             complement(8548..9078)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(9267..9295)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     repeat_region   9341..9378
                     /rpt_family="DR-1"
     terminator      complement(9991..10034)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"