pDonrCbx vector (V007935)

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LOCUS       V007935                 6776 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007935
VERSION     V007935
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold
            SE.
  TITLE     DelsGate, a robust and rapid gene deletion construction method
  JOURNAL   Fungal Genet. Biol. 45 (4), 379-388 (2008)
   PUBMED   18248826
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Garcia-Pedrajas MD, Nadal M, Kapa LB, Perlin MH, Andrews DL, Gold
            SE.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2007) Plant Pathology, University of Georgia,
            Miller Plant Sciences Bldg., Athens, GA 30602, USA
REFERENCE   3  (bases 1 to 6776)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6776)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal
            Genet. Biol."; date: "2008"; volume: "45"; issue: "4"; pages:
            "379-388"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-DEC-2007) Plant Pathology, University of Georgia, Miller Plant
            Sciences Bldg., Athens, GA 30602, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(192..278)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    300..324
                     /label="DonrF primer"
                     /note="DonrF primer"
     protein_bind    332..563
                     /label="attP1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(962..1264)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(1609..2265)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2266..2368)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2513..2744)
                     /label="attP2"
                     /note="recombination site for the Gateway(R) BP reaction
                     (pDONR(TM)221 version)"
     misc_feature    complement(2769..2792)
                     /label="DonrR primer"
                     /note="DonrR primer"
     CDS             2868..3674
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3820..4408
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             5589..6473
                     /gene="SDH2"
                     /label="Succinate dehydrogenase [ubiquinone] iron-sulfur
                     subunit, mitochondrial"
                     /note="Succinate dehydrogenase [ubiquinone] iron-sulfur
                     subunit, mitochondrial from Ustilago maydis (strain 521 /
                     FGSC 9021). Accession#: P32420"