pDOJHR vector (V007938)

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LOCUS       40924_15015        8596 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Shuttle vector pDOJHR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Lee JH, O'sullivan DJ.
  TITLE     Sequence Analysis of Two Cryptic Plasmids from Bifidobacterium 
            longum DJO10A and Construction of a Shuttle Cloning Vector
  JOURNAL   Appl. Environ. Microbiol. 72 (1), 527-535 (2006)
  PUBMED    16391088
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Lee J-H., O'Sullivan DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JUL-2005) Food Science and Nutrition, University of 
            Minnesota, 1500 Gortner Ave, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2006"; volume: "72"; issue: "1"; pages:
            "527-535"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-JUL-2005) Food Science and Nutrition, University of Minnesota, 
            1500 Gortner Ave, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1870..2328)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=cat
                     /note="chloramphenicol resistance"
                     /protein_id="AAZ32757.1"
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPEKCFFSFYYSMDFIYWV"
     gene            complement(1870..2328)
                     /gene="cat"
                     /label=cat
     CDS             2791..2802
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     promoter        complement(4082..4186)
                     /label=AmpR promoter
     primer_bind     4660..4676
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4680..4736)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4746..4762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4770..4786)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4794..4824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4839..4860)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        complement(5313..5341)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     rep_origin      5453..5998
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(6200..7678)
                     /codon_start=1
                     /gene="mob"
                     /product="mobilization protein"
                     /label=mob
                     /protein_id="AAZ32759.1"
                     /translation="MAIYSLNISSASSAVAALSYITSMRVRDDARGETYSGFGRRERVA
                     HVATLLPEGAPSGYADPERLFNAAQAAERGTGVAAKKIMVALPRELDEGQRVLAVERFI
                     RENLTAGGYAATYAIHLDREGRNPHAHILVANRRIDPRTGEWARLKQKTTFALDGNGQR
                     IPVIDPKTGVQKVDKRNRKQWKRVTVSENPLGTKAMLLSMRESWADVCNGLLPEGVRID
                     HRSLEEQGIDRVPTIHEGYASREMEKRGQPSDRMAINREIGASNRDLAEGDAAVDDLRA
                     HQSELRRLLRDMADRAVQAFDRMLESIGHGVDPSDAVEAMRDRDGINLMIPRNAKDSDR
                     PVWHAWLYDRHEWAAIPSHVLREGMRTVGRVRAAIGTALHRWRDAQAARRERIARSTPA
                     QAPRLADVARQAREDAGQPAHQPRPVRKPEQQPEQQRERPVSLAAAAKEASKQLKREQQ
                     QEPEPEPALEWNPWDPADPMNLGMGGSQGYGLGL"
     gene            complement(6200..7678)
                     /gene="mob"
                     /label=mob
     CDS             8011..8493
                     /codon_start=1
                     /gene="orfIV"
                     /product="hypothetical protein"
                     /label=orfIV
                     /protein_id="AAZ32760.1"
                     /translation="MIEPIGGNEMPKSFAQQIEDDENKIKRIREHQRMVRAKQAKQERN
                     ARTKRLVETGAIVEKAHGGAYDDEGRQTFSDGLNGIISVYDPSRGGNVDMRVIDVIDRR
                     IPRLPRSETTTGTAAAASRTVQATAPQPAHAQPQSFTPNPQRIEHRTGAQQPDRWA"
     gene            8011..8493
                     /gene="orfIV"
                     /label=orfIV