pDOE-20 vector (V007940)

LOCUS       V007940                11922 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007940
VERSION     V007940
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 11922)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 11922)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11922)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     regulatory      1328..1965
                     /note="UBQ10; Arabidopsis ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 1481
                     /replace="g"
                     /label="killed PmlI (CACcTG)"
                     /note="killed PmlI (CACcTG)"
     misc_feature    1662..1965
                     /label="cryptic intron from Norris et al. 1993"
                     /note="cryptic intron from Norris et al. 1993"
     misc_difference 1894
                     /replace="t"
                     /label="killed XbaI (aCTAGA)"
                     /note="killed XbaI (aCTAGA)"
     primer_bind     1966..1990
                     /label="pDOE mcs1F with XhoI"
                     /note="pDOE mcs1F with XhoI"
     misc_feature    1978..1994
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2005..2059
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2061..2777
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     misc_feature    2778..2831
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    2832..2853
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      2864..3571
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     regulatory      3592..4229
                     /note="UBQ10; ubiquitin10 promoter"
                     /regulatory_class="promoter"
     misc_difference 3745
                     /replace="g"
                     /label="killed PmlI (CACcTG)"
                     /note="killed PmlI (CACcTG)"
     misc_difference 4158
                     /replace="t"
                     /label="killed XbaI (aCTAGA)"
                     /note="killed XbaI (aCTAGA)"
     primer_bind     4230..4254
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    4237..4252
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      4253..4318
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    4263..4317
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             4319..5035
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     misc_feature    5036..5101
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     misc_feature    5102..5119
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      5121..5373
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5383..5399
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(5424..5440)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5643..5667
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6968..7594
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8026..9096
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9165..9359
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9703..9843
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10029..10617)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10707..11498)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"