pDOE-15 vector (V007945)

LOCUS       V007945                13271 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V007945
VERSION     V007945
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Significant reduction of BiFC non-specific assembly facilitates in
            planta assessment of heterotrimeric G-protein interactors
  JOURNAL   Plant J. (2014) In press
   PUBMED   25187041
REFERENCE   2  (bases 1 to 13271)
  AUTHORS   Gookin TE, Assmann SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2014) Department of Biology, The Pennsylvania
            State University, 208 Mueller Laboratory, University Park, PA 16802,
            USA
REFERENCE   3  (bases 1 to 13271)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13271)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.
            (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-SEP-2014) Department of Biology, The Pennsylvania State
            University, 208 Mueller Laboratory, University Park, PA 16802, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    99..104
                     /label="non-unique SacI"
                     /note="non-unique SacI"
     terminator      complement(111..363)
                     /label="MAS terminator"
                     /note="mannopine synthase terminator"
     misc_feature    complement(373..406)
                     /label="BAR gene remnant"
                     /note="BAR gene remnant"
     misc_feature    407..412
                     /label="MCS2 KpnI site"
                     /note="MCS2 KpnI site"
     CDS             complement(416..931)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(935..1315)
                     /label="MAS promoter"
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2311..2656
                     /label="CaMV35S(short)"
                     /note="Cauliflower mosaic virus 35S promoter (short)"
     misc_feature    2659..2675
                     /label="XhoSac linker"
                     /note="XhoSac linker"
     misc_feature    2686..2740
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2742..3458
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     misc_feature    3459..3512
                     /label="MCS1 front end"
                     /note="MCS1 front end"
     misc_feature    3513..3534
                     /label="MCS1 back end"
                     /note="MCS1 back end"
     terminator      3545..4252
                     /label="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    4261..4266
                     /label="non-unique NdeI"
                     /note="non-unique NdeI"
     promoter        5253..5598
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5594..5618
                     /label="pDOE mcs3bF with Bsu36I"
                     /note="pDOE mcs3bF with Bsu36I"
     misc_feature    5601..5616
                     /label="Bsu361 linker"
                     /note="Bsu361 linker"
     regulatory      5617..5682
                     /label="TMV-Omega enhancer"
                     /note="TMV-Omega enhancer"
                     /regulatory_class="enhancer"
     misc_feature    5627..5681
                     /label="TMV Omega"
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     misc_feature    5683..5730
                     /gene="X-mVenus"
                     /label="MCS3 front end"
                     /note="MCS3 front end"
     CDS             5731..6447
                     /label="mVenus"
                     /note="Venus YFP with monomerizing A206K mutation (Nagai et
                     al., 2002; Kremers et al., 2006)"
     misc_feature    6451..6468
                     /label="MCS3 back end"
                     /note="MCS3 back end"
     terminator      6470..6722
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6732..6748
                     /label="remnant from pFGC5941"
                     /note="remnant from pFGC5941"
     primer_bind     complement(6773..6789)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    6992..7016
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8317..8943
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             9375..10445
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      10514..10708
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11052..11192
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11378..11966)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12056..12847)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"