pDM360 vector (V007970)

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LOCUS       V007970                 8059 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V007970
VERSION     V007970
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Veltman DM, Keizer-Gunnink I, Haastert PJ.
  TITLE     An extrachromosomal, inducible expression system for Dictyostelium
            discoideum
  JOURNAL   Plasmid 61 (2), 119-125 (2009)
   PUBMED   19046986
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Veltman DM, Keizer I, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2008) Cell Biochemistry, Rijks Universiteit
            Groningen, Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 8059)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8059)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2009"; volume: "61"; issue: "2"; pages: "119-125"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen,
            Kerklaan 30, Haren, Groningen 9751 NN, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..298
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     regulatory      473..734
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     regulatory      complement(741..995)
                     /label="mhcA terminator"
                     /note="mhcA terminator"
                     /regulatory_class="terminator"
     CDS             complement(1005..1424)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     regulatory      complement(1436..1657)
                     /label="act6 promoter"
                     /note="act6 promoter"
                     /regulatory_class="promoter"
     promoter        1712..1816
                     /label="AmpR promoter"
     CDS             1817..2674
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2848..3436
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      3649..3932
                     /label="act15 promoter"
                     /note="act15 promoter"
                     /regulatory_class="promoter"
     CDS             3945..4565
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             4587..4820
                     /label="VP16 AD"
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et
                     al., 1989)"
     regulatory      4862..5123
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     rep_origin      complement(5248..5790)
                     /direction=LEFT
                     /label="Ddp1 ori"
                     /note="Ddp1 ori"
     CDS             5700..6293
                     /codon_start=1
                     /product="G5"
                     /label="G5"
                     /protein_id="ACG80845.1"
                     /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNII
                     NKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDKEK
                     DKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLHKKG
                     NPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK"
     CDS             complement(6301..7065)
                     /codon_start=1
                     /product="D5"
                     /label="D5"
                     /protein_id="ACG80846.1"
                     /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCVE
                     KSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDEFG
                     ESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHITNI
                     TQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLDGSNN
                     GMEKSVTQEKNISNNLKINIYLIFFLIIFLIK"
     CDS             complement(7568..8059)
                     /codon_start=1
                     /product="D1'"
                     /label="D1'"
                     /protein_id="ACG80847.1"
                     /translation="MDFEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDF
                     FLVSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIY
                     NVVINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKTN
                     "