pDM326 vector (V007974)

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LOCUS       V007974                 6431 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V007974
VERSION     V007974
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, Van Haastert PJ.
  TITLE     A new set of small, extrachromosomal expression vectors for
            Dictyostelium discoideum
  JOURNAL   Plasmid 61 (2), 110-118 (2009)
   PUBMED   19063918
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Veltman DM, Akar G, Bosgraaf L, van Haastert PJM.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2008) Cell Biochemistry, Rijks Universiteit
            Groningen, Kerklaan 30, Haren 9751 NN, The Netherlands
REFERENCE   3  (bases 1 to 6431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2009"; volume: "61"; issue: "2"; pages: "110-118"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-JUL-2008) Cell Biochemistry, Rijks Universiteit Groningen,
            Kerklaan 30, Haren 9751 NN, The Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      14..303
                     /label="act15 promoter"
                     /note="act15 promoter"
                     /regulatory_class="promoter"
     regulatory      332..594
                     /label="act8 terminator"
                     /note="act8 terminator"
                     /regulatory_class="terminator"
     CDS             606..1091
                     /codon_start=1
                     /product="D1'"
                     /label="D1'"
                     /protein_id="ACH81556.1"
                     /translation="FEKVNSNYYKIQSESTQINSNEIADLKKFIKEEVNKTSSKIDFFL
                     VSSTDALSNPENYSLLEVKCINCHSLCQGKNLYISCTRDGCQNNICYNCLGININIYNV
                     VINSKLCPPCFNDSVINKKCAMCSKNGTKCNLNQECKLHLCAQCSKKCLYILRVKTN"
     CDS             1594..2358
                     /codon_start=1
                     /product="D5"
                     /label="D5"
                     /protein_id="ACH81557.1"
                     /translation="MYSTTLLFFLFFFIVSKSSVLQNDQIEVKYFQLLIVMYQKENCVE
                     KSMTGAVIYDECNIHGRVETNSTHALFYDDIETNNSRCNNFRNLTNLIKLNECINDEFG
                     ESILYKEYNETDDGYLFRVEDSFVEITSLSMDCTKNSKTIIEKFNICSKFENVYHITNI
                     TQEKSNRFTCTDPLCHYCKNENIQNNLDFKTTKCTPKYGASDSEFLSTIYNPKLDGSNN
                     GMEKSVTQEKNISNNLKINIYLIFFLIIFLIK"
     CDS             complement(2366..2959)
                     /codon_start=1
                     /product="G5"
                     /label="G5"
                     /protein_id="ACH81558.1"
                     /translation="MFNHIKDSGYYATNEEIEIFLESCTLCKEITAQTKRNSYKKRNII
                     NKLPEEEEEEEEEEEEEEEEEEQEEEVEKPTISEEEEEETPAVSEEEKEEEEQEEDKEK
                     DKEKKIEEDTETGKKKDEVMNERQDGIEISEKTNDKPTEKTKVKKNVSRVKDINLHKKG
                     NPITPKKIKNFSQQQLRLASGVSNEKRAIITLKK"
     rep_origin      2869..3411
                     /label="Ddp1 ori"
                     /note="Ddp1 ori"
     rep_origin      complement(3742..4330)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4504..5361)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5362..5466)
                     /label="AmpR promoter"
     regulatory      complement(5513..5768)
                     /label="mhcA terminator"
                     /note="mhcA terminator"
                     /regulatory_class="terminator"
     CDS             complement(5778..6197)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     regulatory      complement(6209..6430)
                     /label="act6 promoter"
                     /note="act6 promoter"
                     /regulatory_class="promoter"