pDIVA vector (V007992)

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LOCUS       40924_14725        4311 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pDIVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Construction of infectious full-length cDNA clones of Beet 
            soil-borne mosaic virus and Beet necrotic yellow vein virus A-type 
            by isothermal in vitro recombination analysis of systemic movement, 
            vector transmissions and transreplication properties
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Laufer M, Mohammad H, Varrelmann M, Maiss E.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2016) Phytomedicine, Leibniz Universitaet Hannover
            - Institute of Horticultural Production Systems, Herrenhaeuser 
            Strasse 2, Hannover 30419, Germany
REFERENCE   3  (bases 1 to 4311)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-AUG-2016) Phytomedicine, Leibniz Universitaet Hannover - 
            Institute of Horticultural Production Systems, Herrenhaeuser Strasse
            2, Hannover 30419, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method :: CLC Main Workbench version 6.8.1 Sequencing 
            Technology      :: Sanger dideoxy sequencing ##Assembly-Data-END## 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..622
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     CDS             839..1630
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             1932..3077
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    complement(3235..3259)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        3412..3754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3755..3839
                     /label=HDVagrz
                     /note="HDVagrz"
     misc_feature    3840..3874
                     /label=35 bp spacer
                     /note="35 bp spacer"
     misc_feature    3875..4079
                     /label=pA35S
                     /note="pA35S"
     primer_bind     complement(4109..4125)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(4206..4230)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      4310..4311
                     /label=oriV
                     /note="incP origin of replication"