pDGO144 vector (V008000)

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LOCUS       V008000                 4475 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008000
VERSION     V008000
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Development of a plasmid-based expression system in Clostridium
            thermocellum and its use to screen heterologous expression of
            bifunctional alcohol dehydrogenases (adhEs)
  JOURNAL   Metab Eng Commun 3, 120-129 (2016)
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Hon S, Lanahan AA, Tian L, Giannone RJ, Hettich RL, Olson DG, Lynd
            LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 4475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng
            Commun 3, 120-129 (2016)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1002
                     /label="repB"
                     /note="RepB replication protein"
     misc_feature    1011..1051
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     regulatory      1088..1179
                     /gene="cat"
                     /regulatory_class="promoter"
     CDS             1180..1827
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1911..2768)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2769..2860)
                     /label="AmpR promoter"
     rep_origin      3460..4005
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."