pDGO126'ins'_p2638-1705 vector (V008003)

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LOCUS       V008003                 5412 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008003
VERSION     V008003
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in
            Thermoanaerobacterium saccharolyticum and its role in ethanol
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27694237
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(68..925)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(926..1017)
                     /label="AmpR promoter"
     rep_origin      1617..2162
                     /direction=RIGHT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     CDS             2633..3634
                     /label="repB"
                     /note="RepB replication protein"
     regulatory      3679..3887
                     /label="P2638"
                     /note="P2638"
                     /regulatory_class="promoter"
     CDS             3888..4619
                     /codon_start=1
                     /product="putative ferredoxin:NAD+ oxidoreductase"
                     /EC_number="1.18.1.2"
                     /label="putative ferredoxin:NAD+ oxidoreductase"
                     /note="Tsac_1705"
                     /protein_id="AOU74527.1"
                     /translation="MRYVVRENREISNGIYRLRLEFDGIPTPGQFIMINCGGNTLLKRP
                     FSICDHDADEITIVYRIKGKGTYNLSKVKALDTLEVTGPHGHGFDMFDKFNNILIVGGG
                     IGIPPLLFLSKKLKSKNLKIALGFKSDVFLVDEFKKYGDVVIATEDGNTGIKGYVTDAM
                     KDEIKDIDMIYGCGPMQMLKSVKDMAEKYDVPCQISIEEHMGCGIGACMVCACKTKTDS
                     GFQYKKVCKDGPVFWSREVVF"
     CDS             4749..5396
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"