pDGO-34 vector (V008008)

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LOCUS       V008008                10309 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008008
VERSION     V008008
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Waller BH, Olson DG, Currie DH, Guss AM, Lynd LR.
  TITLE     Exchange of type II dockerin-containing subunits of the Clostridium
            thermocellum cellulosome as revealed by SNAP-tags
  JOURNAL   FEMS Microbiol. Lett. 338 (1), 46-53 (2013)
   PUBMED   23082914
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   Olson DG.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2012) Thayer School of Engineering, Dartmouth
            College, Thayer School of Engineering, Dartmouth College, Hanover,
            NH 03755, USA
REFERENCE   3  (bases 1 to 10309)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10309)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2013"; volume: "338"; issue: "1"; pages:
            "46-53"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-AUG-2012) Thayer School of Engineering, Dartmouth College,
            Thayer School of Engineering, Dartmouth College, Hanover, NH 03755,
            USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..514
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     misc_feature    680..1214
                     /label="5' flank (CipA + promoter)"
                     /note="5' flank (CipA + promoter)"
     misc_feature    1215..1753
                     /label="3' flank"
                     /note="3' flank"
     regulatory      1766..2413
                     /label="gapD promoter from Clostridium thermocellum"
                     /note="gapD promoter from Clostridium thermocellum"
                     /regulatory_class="promoter"
     CDS             2414..3061
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             3082..3627
                     /codon_start=1
                     /gene="hpt"
                     /product="putative hypoxanthine phosphoribosyltransferase"
                     /EC_number="2.4.2.8"
                     /label="hpt"
                     /protein_id="AFU51886.1"
                     /translation="MENLSKDIDEILITEEELKEKIKELGRQITKDYKGKNLMLVGVLK
                     GALMFMADLSRHIDLPLSLDFMAVSSYGSSTHSSGIVKIIKDLDISIEGKDVLIVEDII
                     DSGLTLSYLRETLLGRKPKSLKICTILDKPERREASVKVDYVGFKIPDKFVVGYGLDFD
                     EKYRNLPFIGVLKPEMYS"
     gene            3082..3627
                     /gene="hpt"
                     /label="hpt"
     terminator      4305..4552
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(4800..5388)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5475..6053)
                     /codon_start=1
                     /gene="tdk"
                     /product="thymidine kinase"
                     /EC_number="2.7.1.21"
                     /label="tdk"
                     /protein_id="AFU51887.1"
                     /translation="MYGPKDHGYIEVVTGPMFSGKSEELIRRIKRAKIARQKVQVFKPA
                     IDDRYSIDKVVSHNGDNMHAIAIVKASDILAYAEEDTDVFAIDEVQFFDSEIVDIVKEI
                     ADSGKRVICAGLDMDFRGEPFGPTPELMAIAEFVDKLTAICMKCGNPATRTQRLINGKP
                     ANYDDPIIMVGAKESYEARCRKCHEVPRT"
     gene            complement(5475..6053)
                     /gene="tdk"
                     /label="tdk"
     regulatory      complement(6054..6674)
                     /note="CBP; cellulose-binding protein promoter"
                     /regulatory_class="promoter"
     CDS             complement(6753..7754)
                     /label="repB"
                     /note="RepB replication protein"
     CDS             complement(8315..9172)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(9271..10071)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(10072..10292)
                     /label="URA3 promoter"