pDG4 vector (V008012)

LOCUS       V008012                 8189 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008012
VERSION     V008012
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, Kuo MH.
  TITLE     A tethered catalysis, two-hybrid system to identify protein-protein
            interactions requiring post-translational modifications
  JOURNAL   Nat. Biotechnol. 22 (7), 888-892 (2004)
   PUBMED   15208639
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Kuo M-H., Guo D.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUN-2004) Biochem. Mol. Biol., Michigan State
            University, 309 BCH Building, East Lansing, MI 48824, USA
REFERENCE   3  (bases 1 to 8189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2004"; volume: "22"; issue: "7"; pages:
            "888-892"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-JUN-2004) Biochem. Mol. Biol., Michigan State University, 309
            BCH Building, East Lansing, MI 48824, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             434..874
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             944..1018
                     /note="Histone H4 from Medicago sativa. Accession#: P62789"
                     /label="Histone H4"
     CDS             1097..1108
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             1850..1939
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     terminator      2378..2565
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(3397..4559)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(5073..5174)
                     /label="TRP1 promoter"
     promoter        5280..5384
                     /label="AmpR promoter"
     CDS             5385..6242
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6416..7004
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"