pDG3661 vector (V008013)

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LOCUS       V008013                10407 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V008013
VERSION     V008013
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     rRNA promoter strength and regulation in Bacillus subtilis: an
            alternative strategy for the modulation of ribosome synthesis in
            bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Krasny L, Gourse RL.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Bacteriology, University of Wisconsin,
            Madison, 420 Henry Mall, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 10407)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10407)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2004) Bacteriology, University of Wisconsin, Madison, 420
            Henry Mall, Madison, WI 53706, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            115..635
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE front; partial alpha-amylase; similar to
                     amyE-front in GenBank Accession Number U46196"
     regulatory      751..837
                     /note="derived from Bacillus subtilis rrnB; terminates
                     transcription from amyE front"
                     /regulatory_class="terminator"
     regulatory      complement(844..932)
                     /note="derived from Bacillus subtilis rrnO; terminates lacZ
                     transcription"
                     /regulatory_class="terminator"
     CDS             complement(950..3982)
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             4391..5038
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     gene            5412..6440
                     /gene="amyE"
                     /label="amyE"
                     /note="amyE back; partial alpha-amylase; similar to
                     amyE-back in GenBank Accession Number U46196"
     CDS             7549..8328
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     rep_origin      complement(8578..9166)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9340..10197)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10198..10302)
                     /label="AmpR promoter"