Basic Vector Information
- Vector Name:
- pDG1i-tdTomato-V5-Blast
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9809 bp
- Type:
- Lentiviral vector
- Replication origin:
- ori
- Source/Author:
- De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I, Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele P, Haustraete J, Declercq W.
- Promoter:
- RSV
pDG1i-tdTomato-V5-Blast vector Map
pDG1i-tdTomato-V5-Blast vector Sequence
LOCUS V008024 9809 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V008024
VERSION V008024
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9809)
AUTHORS De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I,
Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele
P, Haustraete J, Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60 (5), 252-259 (2016)
PUBMED 27177818
REFERENCE 2 (bases 1 to 9809)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2015) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 9809)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9809)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "2016"; volume: "60"; issue: "5"; pages:
"252-259"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-DEC-2015) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9809
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 35..159
/label="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 184..214
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 268..924
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 1269..1571
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(1615..1739)
/label="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 1771..3198
/label="tdTomato"
/note="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
CDS 3217..3258
/codon_start=1
/product="epitope tag from simian virus 5"
/label="V5 tag"
/translation="GKPIPNPLLGLDST"
promoter 3300..3630
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
promoter 3678..3725
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS 3744..4139
/label="BSD"
/note="blasticidin S deaminase"
misc_feature 4212..4227
/label="HIV-1 cPPT"
/note="HIV-1 cPPT"
LTR 4229..4462
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4534..4668
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 4695..4830
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(4851..4869)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4879..4895)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 5037..5492
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5518..5622
/label="AmpR promoter"
CDS 5623..6480
/label="AmpR"
/note="beta-lactamase"
rep_origin 6654..7242
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7530..7551
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7566..7596
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 7604..7620
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7628..7644
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 7665..7683
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 7711..7937
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 7938..8118
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8165..8290
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8783..9016
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9201..9245
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 9394..9435
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
protein_bind 9533..9551
/label="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9568..9586
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9604..9622
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9640..9658
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9675..9693
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9711..9729
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 9741..9779
/label="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
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