Basic Vector Information
- Vector Name:
- pDG1i-eGFP-V5-Blast
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9098 bp
- Type:
- Lentiviral vector
- Replication origin:
- ori
- Source/Author:
- De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I, Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele P, Haustraete J, Declercq W.
- Promoter:
- RSV
pDG1i-eGFP-V5-Blast vector Vector Map
pDG1i-eGFP-V5-Blast vector Sequence
LOCUS V008026 9098 bp DNA circular SYN 18-DEC-2018
DEFINITION Exported.
ACCESSION V008026
VERSION V008026
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 9098)
AUTHORS De Groote P, Grootjans S, Lippens S, Eichperger C, Leurs K, Kahr I,
Tanghe G, Bruggeman I, De Schamphelaire W, Urwyler C, Vandenabeele
P, Haustraete J, Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60 (5), 252-259 (2016)
PUBMED 27177818
REFERENCE 2 (bases 1 to 9098)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (22-DEC-2015) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 9098)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9098)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "2016"; volume: "60"; issue: "5"; pages:
"252-259"
SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-DEC-2015) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9098
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 35..159
/label="attR1"
/note="recombination site for the Gateway(R) LR reaction"
promoter 184..214
/label="lac UV5 promoter"
/note="E. coli lac promoter with an 'up' mutation"
CDS 268..924
/label="CmR"
/note="chloramphenicol acetyltransferase"
CDS 1269..1571
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(1615..1739)
/label="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 1771..2487
/label="EGFP"
/note="enhanced GFP"
CDS 2506..2547
/codon_start=1
/product="epitope tag from simian virus 5"
/label="V5 tag"
/translation="GKPIPNPLLGLDST"
promoter 2589..2919
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
promoter 2967..3014
/label="EM7 promoter"
/note="synthetic bacterial promoter"
CDS 3033..3428
/label="BSD"
/note="blasticidin S deaminase"
misc_feature 3501..3516
/label="HIV-1 cPPT"
/note="HIV-1 cPPT"
LTR 3518..3751
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3823..3957
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 3984..4119
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(4140..4158)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4168..4184)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4326..4781
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4807..4911
/label="AmpR promoter"
CDS 4912..5769
/label="AmpR"
/note="beta-lactamase"
rep_origin 5943..6531
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6819..6840
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6855..6885
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6893..6909
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6917..6933
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6954..6972
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 7000..7226
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
LTR 7227..7407
/label="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7454..7579
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8072..8305
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8490..8534
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 8683..8724
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
protein_bind 8822..8840
/label="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8857..8875
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8893..8911
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8929..8947
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 8964..8982
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 9000..9018
/gene="tetO"
/label="tet operator"
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
promoter 9030..9068
/label="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
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