pdEYFP vector (V008028)

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LOCUS       40924_14505        5935 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian vector pdEYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5935)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5935)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    591..670
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    675..799
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        824..854
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             908..1564
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1909..2211
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2255..2379)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2396..3112
                     /label=EYFP
                     /note="enhanced YFP"
     polyA_signal    3238..3359
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3366..3821)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3848..3952
                     /label=AmpR promoter
     promoter        3954..4311
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      4319..4371
                     /label=lacUV5 promoter
                     /note="lacUV5 promoter"
                     /regulatory_class="promoter"
     promoter        4336..4366
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4409..4939
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      5233..5821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"