pDESTR vector (V008029)

LOCUS       40924_14490        5768 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pDESTR DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Construction of pDESTR, a GATEWAY vector for gene disruption in 
            filamentous fungi
  JOURNAL   Curr. Microbiol. 52 (3), 210-215 (2006)
  PUBMED    16479358
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Abe A, Elegado EB, Sone T.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JUN-2005) Teruo Sone, Graduate School of Agriculture, 
            Hokkaido University, Laboratory of Applied Microbiology; Kita-9 
            Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 
            (E-mail:sonet@chem.agr.hokudai.ac.jp, Tel:81-11-706-2502, 
            Fax:81-11-706-4961)
REFERENCE   3  (bases 1 to 5768)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5768)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2006"; volume: "52"; issue: "3"; pages: 
            "210-215"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-JUN-2005) Teruo Sone, Graduate School of Agriculture, Hokkaido 
            University, Laboratory of Applied Microbiology"; volume: " Kita-9 
            Nishi-9, Kita-ku, Sapporo, Hokkaido 060-8589, Japan 
            (E-mail:sonet@chem.agr.hokudai.ac.jp, Tel:81-11-706-2502, Fax"; 
            pages: "81-11-706-4961"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5768
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      26..481
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        559..663
                     /label=AmpR promoter
     CDS             664..1521
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1695..2283
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2564..2918
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             2923..3945
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    4060..4184
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4209..4239
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             4293..4949
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             5294..5596
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(5640..5764)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"