Basic Vector Information
- Vector Name:
- pDEST152
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11519 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Wu FL, Liu Y, Zhang HN, Jiang HW, Cheng L, Guo SJ, Deng JY, Bi LJ, Zhang XE, Gao HF, Tao SC.
- Promoter:
- CMV
pDEST152 vector Vector Map
pDEST152 vector Sequence
LOCUS 40924_14425 11519 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pDEST152, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11519)
AUTHORS Wu FL, Liu Y, Zhang HN, Jiang HW, Cheng L, Guo SJ, Deng JY, Bi LJ,
Zhang XE, Gao HF, Tao SC.
TITLE Global profiling of PknG interactions using a human proteome
microarray reveals novel connections with CypA
JOURNAL Proteomics, e1800265 (2018) In press
PUBMED 30281201
REFERENCE 2 (bases 1 to 11519)
AUTHORS Wu FL, Tao SC.
TITLE Direct Submission
JOURNAL Submitted (22-MAR-2018) Shanghai Center for Systems Biomedicine,
Shanghai Jiao Tong University, Dongchuan Road, No. 800, Shanghai,
Shanghai 200210, China
REFERENCE 3 (bases 1 to 11519)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11519)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proteomics,
e1800265 (2018) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-MAR-2018) Shanghai Center for Systems Biomedicine, Shanghai Jiao
Tong University, Dongchuan Road, No. 800, Shanghai, Shanghai 200210,
China"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..11519
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 475..2264
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(2573..4495)
/codon_start=1
/label=EBNA1
/note="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
promoter 5016..5120
/label=AmpR promoter
CDS 5121..5978
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6152..6740
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 6968..7265
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 7343..7939
/codon_start=1
/label=PuroR
/note="puromycin N-acetyltransferase"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
polyA_signal 8033..8167
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
enhancer 8426..8805
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8806..9009
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 9178..9195
/codon_start=1
/label=thrombin site
/note="thrombin recognition and cleavage site"
/translation="LVPRGS"
protein_bind 9208..9332
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 9357..9387
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 9441..10097
/codon_start=1
/label=CmR
/note="chloramphenicol acetyltransferase"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 10442..10744
/codon_start=1
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(10788..10912)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
CDS 10920..10937
/codon_start=1
/label=thrombin site
/note="thrombin recognition and cleavage site"
/translation="LVPRGS"
CDS 10938..10961
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
CDS 10968..10985
/codon_start=1
/label=thrombin site
/note="thrombin recognition and cleavage site"
/translation="LVPRGS"
CDS 10989..11012
/codon_start=1
/label=Strep-Tag II
/note="peptide that binds Strep-Tactin(R), an engineered
form
of streptavidin"
/translation="WSHPQFEK"
CDS 11049..11072
/codon_start=1
/label=Strep-Tag II
/note="peptide that binds Strep-Tactin(R), an engineered
form
of streptavidin"
/translation="WSHPQFEK"
CDS 11082..11105
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
polyA_signal complement(11110..11244)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
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