pDEST-N-EYFP vector (V008042)

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LOCUS       40924_14405        5984 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Diatom vector pDEST-N-EYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5984)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5984)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(6..461)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      865..1100
                     /label=FcpB promoter
                     /note="FcpB promoter"
                     /regulatory_class="promoter"
     CDS             1108..1824
                     /label=EYFP
                     /note="enhanced YFP"
     protein_bind    1837..1961
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1986..2016
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2070..2726
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3071..3373
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(3417..3541)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      3709..3780
                     /label=FcpA terminator
                     /note="FcpA terminator"
                     /regulatory_class="terminator"
     promoter        complement(3799..3817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3838..3854)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3862..3878)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3886..3916)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3931..3952)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4240..4828)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5002..5859)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5860..5964)
                     /label=AmpR promoter