Basic Vector Information
- Vector Name:
- pDEST-Myc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6078 bp
- Type:
- Mammalian vector
- Replication origin:
- ori
- Source/Author:
- De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman Fonseca M, Vanhoucke M, Beyaert R.
- Promoter:
- sCMV
pDEST-Myc vector Map
pDEST-Myc vector Sequence
LOCUS 40924_14400 6078 bp DNA circular SYN 18-DEC-2018
DEFINITION Mammalian vector pDEST-Myc, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6078)
AUTHORS De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
Fonseca M, Vanhoucke M, Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6078)
AUTHORS De Schamphelaire W.
TITLE Direct Submission
JOURNAL Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM
REFERENCE 3 (bases 1 to 6078)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6078)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
9052, BELGIUM"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6078
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory complement(1..17)
/label=T7 promoter
/note="T7 promoter"
/regulatory_class="promoter"
polyA_signal complement(22..156)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(269..287)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(308..324)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(332..348)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(356..386)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(401..422)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(710..1298)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1472..2329)
/label=AmpR
/note="beta-lactamase"
promoter complement(2330..2434)
/label=AmpR promoter
rep_origin complement(2460..2915)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2989..3973
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
promoter 4008..4026
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
CDS 4076..4105
/label=Myc
/note="Myc (human c-Myc proto-oncogene) epitope tag"
misc_feature 4115..4144
/label=Myc epitope
/note="Myc epitope"
CDS 4115..4144
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 4154..4183
/label=Myc epitope
/note="Myc epitope"
CDS 4154..4183
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 4193..4222
/label=Myc epitope
/note="Myc epitope"
CDS 4193..4222
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 4232..4261
/label=Myc epitope
/note="Myc epitope"
CDS 4232..4261
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 4295..4324
/label=Myc epitope
/note="Myc epitope"
CDS 4295..4324
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
protein_bind 4352..4476
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
promoter 4501..4531
/label=lac UV5 promoter
/note="E. coli lac promoter with an 'up' mutation"
CDS 4585..5241
/label=CmR
/note="chloramphenicol acetyltransferase"
CDS 5586..5888
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
protein_bind complement(5932..6056)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
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