pDAL649 vector (V008069)

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LOCUS       V008069                 3905 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008069
VERSION     V008069
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Hernday A.
  TITLE     Structure of the Transcriptional Network Controlling White-Opaque
            Switching in Candida albicans
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Hernday A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th
            St, San Francisco, CA 94107, USA
REFERENCE   3  (bases 1 to 3905)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-NOV-2012) Microbiology and Immunology, UCSF, 600 16th St, San
            Francisco, CA 94107, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..32
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             234..263
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    complement(441..460)
                     /label="lac operator (symmetric)"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The
                     symmetric lac operator was optimized for tight binding of
                     lac repressor."
     protein_bind    474..490
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             509..520
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             527..1180
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             1211..1906
                     /gene="cpxR"
                     /label="Transcriptional regulatory protein CpxR"
                     /note="Transcriptional regulatory protein CpxR from
                     Escherichia coli (strain K12). Accession#: P0AE88"
     terminator      1923..2009
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2174..2762)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(2850..2944)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2978..3769)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"