pCXneo vector (V008089)

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LOCUS       40924_14015        6248 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral vector pCXneo DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Akagi T, Shishido T, Murata K, Hanafusa H.
  TITLE     v-Crk activates the phosphoinositide 3-kinase/AKT pathway in 
            transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (13), 7290-7295 (2000)
  PUBMED    10852971
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute, 
            The First Department; 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850, 
            Fax:81-6-6871-6686)
REFERENCE   3  (bases 1 to 6248)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "13"; pages: 
            "7290-7295"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-APR-2000) Tsuyoshi Akagi, Osaka Bioscience Institute, The First 
            Department"; volume: " 6-2-4 Furuedai, Suita, Osaka 565-0874, Japan 
            (E-mail:takagi@obisun1.obi.or.jp, Tel:81-6-6872-4850, Fax"; pages: 
            "81-6-6871-6686"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label=R-U5 region of MuLV 5'LTR
                     /note="R-U5 region of MuLV 5'LTR"
     misc_feature    826..1183
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1248..1664
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1674..2048
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2052..2136
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    2187..2748
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             2751..3542
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     LTR             3605..4198
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4435..5023)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5190..6047)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6048..6152)
                     /label=AmpR promoter