pCX4.1hisD vector (V008100)

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LOCUS       V008100                 6690 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008100
VERSION     V008100
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Akagi T, Sasai K, Hanafusa H.
  TITLE     Refractory nature of normal human diploid fibroblasts with respect
            to oncogene-mediated transformation
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 100 (23), 13567-13572 (2003)
   PUBMED   14597713
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Akagi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute,
            Molecular Oncology; Furuedai 6-2-4, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax:81-6-6871-7521)
REFERENCE   3  (bases 1 to 6690)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2003"; volume: "100"; issue: "23"; pages:
            "13567-13572"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-JUN-2002) Tsuyoshi Akagi, Osaka Bioscience Institute, Molecular
            Oncology"; volume: " Furuedai 6-2-4, Suita, Osaka 565-0874, Japan
            (E-mail:takagi@obi.or.jp, Tel:81-6-6872-4834, Fax"; pages:
            "81-6-6871-7521"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        88..467
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   467..762
                     /label="R-U5 region of MuLV 5' LTR"
                     /note="R-U5 region of MuLV 5' LTR"
     misc_feature    826..1183
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1242..1658
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1668..2018
                     /label="Mo-MuLV pol region containing splice acceptor"
                     /note="Mo-MuLV pol region containing splice acceptor"
     misc_feature    2019..2080
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     misc_feature    2128..2689
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             2692..3993
                     /gene="hisD"
                     /label="Histidinol dehydrogenase"
                     /note="Histidinol dehydrogenase from Salmonella typhimurium
                     (strain LT2 / SGSC1412 / ATCC 700720). Accession#: P10370"
     LTR             4047..4640
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(4877..5465)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5632..6489)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6490..6594)
                     /label="AmpR promoter"