pCU-LYS21 vector (V008114)

LOCUS       40924_13815        6438 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCU-LYS21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Zordan RE, Ren Y, Pan SJ, Rotondo G, Penas Ade L, Iluore J, Cormack 
            BP.
  TITLE     Expression Plasmids for Use in Candida glabrata
  JOURNAL   G3 (Bethesda) 3 (10), 1675-1686 (2013)
  PUBMED    23934995
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Zordan RE, Cormack BP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAY-2013) Molecular Biology and Genetics, Johns 
            Hopkins School of Medicine, 725 N Wolfe St., Hunterian 609, 
            Baltimore, MD 21205, USA
REFERENCE   3  (bases 1 to 6438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)"; date: "2013"; volume: "3"; issue: "10"; pages: 
            "1675-1686"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-MAY-2013) Molecular Biology and Genetics, Johns Hopkins School 
            of Medicine, 725 N Wolfe St., Hunterian 609, Baltimore, MD 21205, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(66..664)
                     /label=Candida glabrata CEN/ARS
                     /note="Candida glabrata CEN/ARS"
     promoter        666..737
                     /label=AmpR promoter
     CDS             738..1595
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1769..2357
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2645..2666
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2681..2711
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2719..2735
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2743..2759
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2780..2798
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2817..3973
                     /label=Candida glabrata LYS21 promoter
                     /note="Candida glabrata LYS21 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(4024..4040)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      4043..4440
                     /label=region downstream of HIS3 in Candida glabrata BG2
                     /note="region downstream of HIS3 in Candida glabrata BG2"
                     /regulatory_class="terminator"
     promoter        complement(4455..4473)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4483..4499)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4640..5095
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(5229..6029)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(6030..6245)
                     /label=URA3 promoter